Method for preparing ultra-low molecular weight dendrobium oligosaccharides by enzymatic hydrolysis and its application

Abstract

The disclosure provides a method for preparing ultra-low molecular weight Dendrobium oligosaccharides by enzymatic hydrolysis and its application, and belongs to the technical field of bioengineering. In the disclosure, Thermobifida halotolerans-derived glycosidases are recombined to an Escherichia coli expression system to realize high activity expression. The recombinantly expressed ThDPS enzyme can hydrolyze Dendrobium polysaccharides, and produced oligosaccharide products mainly include ultra-low molecular weight (<1000 Daltons) Dendrobium disaccharides, Dendrobium trisaccharides, Dendrobium tetrasaccharides and Dendrobium pentasaccharides, and low-molecular weight (<10000 Daltons) Dendrobium hexaoses, Dendrobium heptaoses and Dendrobium octacoses, and higher Dendrobium oligosaccharide products. The disclosure further provides application of Dendrobium oligosaccharides prepared by enzymatic hydrolysis in promoting proliferation of lactic acid bacteria, laying a technical foundation for the biological preparation of the ultra-low molecular weight Dendrobium oligosaccharides by enzymatic hydrolysis and the development of prebiotics, and being suitable for industrial production and application.

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