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US 12,735,728 B2Granted
Yeast strain development for ethanol production
AssigneesAB Mauri, Vib Vzw, Katholieke Universiteit Leuven
InventorsJohn H. Evans, IV, Aaron J. McKerracher, Jan Steensels, Kevin J. Verstrepen
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Abstract
Disclosed herein are yeast strains and derivatives thereof, as well as compositions comprising the yeast strains for use in ethanol manufacture. The disclosure also relates to processes for producing ethanol from biomass using the yeast strains and compositions. In particular, the yeast strains produce lower glycerol and higher ethanol, and have a higher temperature tolerance and higher fermentation rate than strains and products currently used in ethanol production processes.
Claims17
A Saccharomyces yeast strain wherein:
- (i) the yeast strain is selected from the group consisting of:
- (a) Saccharomyces strain Y1912, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68003;
- (b) Saccharomyces strain Y1913, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68004;
- (c) Saccharomyces strain Y1914, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68005;
- (d) Saccharomyces strain Y1919, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68006;
- (e) Saccharomyces strain Y1923, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68007;
- (f) Saccharomyces strain Y1927, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68008;
- and,
- (g) Saccharomyces strain Y1929, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68009;
- and
- (ii) the yeast strain comprise one or more defining characteristics selected from the group consisting of:
- (a) a higher ethanol yield than Saccharomyces strain Y1609 under same fermentation conditions;
- (b) an increased temperature tolerance compared to Saccharomyces strain Y1609 at a temperature from 32° C. to 36° C.;
- (c) a lower glycerol yield than Saccharomyces strain Y1609 under same fermentation conditions;
- (d) an increased organic acid tolerance compared to Saccharomyces strain Y1609 at a pH from about 4.0 to about 5.2 in the presence of organic acids;
- and
- (e) an increased fermentation rate compared to Saccharomyces strain Y1609 under the same fermentation conditions.
14 more elements
A process for producing ethanol from fermentable a substrate by contacting the fermentable substrate with a fermenting organism, wherein:
- (i) the fermenting organism is selected from the group consisting of:
- (a) Saccharomyces strain Y1912, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68003;
- (b) Saccharomyces strain Y1913, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68004;
- (c) Saccharomyces strain Y1914, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68005;
- (d) Saccharomyces strain Y1919, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68006;
- (e) Saccharomyces strain Y1923, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68007;
- (f) Saccharomyces strain Y1927, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68008;
- and,
- (g) Saccharomyces strain Y1929, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68009;
- and
- (ii) the fermenting organism comprises one or more defining characteristics selected from the group consisting of:
- (a) a higher ethanol yield than Saccharomyces strain Y1609 under same fermentation conditions;
- (b) an increased temperature tolerance compared to Saccharomyces strain Y1609 at a temperature from 32° C. to 36° C.;
- (c) a lower glycerol yield than Saccharomyces strain Y1609 under same fermentation conditions;
- (d) an increased organic acid tolerance compared to Saccharomyces strain Y1609 at a pH from about 4.0 to about 5.2 in the presence of organic acids;
- and
- (e) an increased fermentation rate compared to Saccharomyces strain Y1609 under the same fermentation conditions.
14 more elements
A method of producing a fermentation product from a fermentable substrate by contacting the fermentable substrate with a fermenting organism, wherein the fermenting organism is selected from the group consisting of:
- (a) Saccharomyces strain Y1912, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68003;
- (b) Saccharomyces strain Y1913, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68004;
- (c) Saccharomyces strain Y1914, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68005;
- (d) Saccharomyces strain Y1919, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68006;
- (e) Saccharomyces strain Y1923, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68007;
- (f) Saccharomyces strain Y1927, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68008;
- and,
- (g) Saccharomyces strain Y1929, a representative sample of the strain having been deposited under NRRL Patent Deposit Designation No. Y-68009.
5 more elements
Dependencies are inferred from the claim text.