Method for isolating highly pure nucleic acid with magnetic particles

Abstract

Subject of the invention is a method for isolating nucleic acid from a sample solution, wherein the nucleic acid is separated from the sample solution by adsorption to magnetic particles, followed by separation of the magnetic particles with the nucleic acid adsorbed thereto from the sample solution by application of a magnetic field, followed by at least one washing step (w), comprising (w1) suspending the magnetic particles with the nucleic acid adsorbed thereto in a washing solution, which is an aqueous solution, which has a total salt concentration below 100 mM and does not comprise an organic solvent, (w2) incubating the magnetic particles with the nucleic acid adsorbed thereto in the washing solution, and (w3) separating the magnetic particles with the nucleic acid adsorbed thereto from the washing solution. The invention also relates to use of an aqueous solution, which has a total salt concentration below 100 mM and does not comprise an organic solvent, as a washing solution for washing magnetic particles with nucleic acid adsorbed thereto.

Dependencies are inferred from the claim text.