RNA-guided nuclease
AssigneeInari Agriculture Technology, Inc.
InventorsNeena Pyzocha, Adam Joyce, Karl Kremling
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Abstract
Compositions and methods related to Cas proteins, nucleic acids encoding the Cas proteins, and modified host cells comprising the Cas proteins and/or encoding nucleic acids are disclosed. Cas proteins are useful in a variety of applications. Cas proteins bind guide RNAs that in turn provide functional specificity to the Cas proteins, nucleic acids encoding the Cas guide RNAs, and modified host cells comprising the Cas guide RNAs and/or encoding nucleic acids. The Cas polypeptides and corresponding guide RNAs can be used in a variety of applications.
Claims4
A CRISPR/Cas endonuclease fusion polypeptide comprising a CRISPR/Cas endonuclease fused to a heterologous polypeptide, wherein the CRISPR/Cas endonuclease comprises an amino acid sequence having 95% or more identity to the amino acid sequence set forth in SEQ ID NO:
- 3, wherein the heterologous polypeptide comprises a nuclear localization signal (NLS), and wherein the CRISPR/Cas endonuclease comprises a RuvC-I domain with an amino acid sequence IXGXDLG (SEQ ID NO:
- 40), a RuvC-II domain with an amino acid sequence IQMEXL (SEQ ID NO:
- 41), and a RuvC-III domain with an amino acid sequence NADXNAXXNI (SEQ ID NO:
- 42).
Dependencies are inferred from the claim text.