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US 12,735,688 B2Granted
Enzymes with RUVC domains
AssigneeMetagenomi Therapeutics, Inc.
InventorsBrian Thomas, Christopher Brown, Rose Kantor, Audra Devoto, Cristina Butterfield, Lisa Alexander, Daniela S. A. Goltsman, Jason Liu
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Abstract
The present disclosure provides for endonuclease enzymes having distinguishing domain features, as well as methods of using such enzymes or variants thereof.
Claims2
An engineered nuclease composition comprising:
- (a) an endonuclease comprising a RuvC_III domain and an HNH domain, wherein said RuvC_III domain comprises a sequence having at least 85% sequence identity to at least 21 consecutive amino acids of SEQ ID NO:
- 2917 and wherein said HNH domain comprises a sequence having at least 85% sequence identity to at least 20 consecutive amino acids of SEQ ID NO:
- 4731;
- and
- (b) an engineered guide ribonucleic acid structure configured to form a complex with said endonuclease comprising:
- (i) a guide ribonucleic acid sequence configured to hybridize to a target deoxyribonucleic acid sequence;
- and
- (ii) a tracr ribonucleic acid sequence configured to bind to said endonuclease.
5 more elements
Dependencies are inferred from the claim text.