Enzymatic production of allulose
AssigneeBonumose, Inc.
InventorDaniel Joseph Wichelecki
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Abstract
The invention relates to improved processes for the enzymatic production of allulose using enzymes which have been characterized as having improved expression, improved stability, and low allulose to fructose conversion activity, relative to enzymes in other allulose production methods. Improved processes include steps of converting fructose-6-phosphate to allulose 6-phopsphate A6P) using an allulose 6-phosphate epimerase, and converting A6P to allulose using an allulose-6-phosphate phosphatase.
Claims19
An improved process for the production of allulose from a saccharide, the improved process comprising the steps of:
- converting fructose-6-phosphate (F6P) to allulose 6-phopsphate (A6P) using an allulose 6-phosphate epimerase (A6PE), wherein the A6PE comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 1;
- and
- converting A6P to allulose using an allulose 6-phosphate phosphatase (A6PP), wherein the improved process produces more allulose than a process for producing allulose under the same process conditions using Bacillus thermoamylovorans A6PE comprising the amino acid sequence as set forth in SEQ ID NO:
- 4.
An improved process for the production of allulose from a saccharide, the improved process comprising the steps of:
- converting fructose-6-phosphate (F6P) to allulose 6-phopsphate (A6P) using an allulose 6-phosphate epimerase (A6PE);
- and
- converting A6P to allulose using an allulose-6-phoshpate phosphatase (A6PP), wherein the A6PP comprises the amino acid sequence as set forth in SEQ ID NO:
- 2,
- wherein the improved process produces more allulose than a process for producing allulose under the same process conditions using Clostridium thermocellum A6PP comprising the amino acid sequence as set forth in SEQ ID NO:
- 10.
Dependencies are inferred from the claim text.