Amorpha-4,11-diene 12-monooxygenase variants and uses thereof
AssigneeAmyris, Inc.
InventorsChristopher J. Paddon, Hanxiao Jiang, Stephanie H. Kung
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Abstract
Provided herein are genetically modified host cells, compositions, and methods for improved production of artemisinic acid. The host cells are genetically modified to contain a heterologous nucleic acid that expresses novel and optimized variants of Amorpha-4,11-diene 12-monooxygenase. Also provided herein are methods for screening for variants of cytochrome p450 enzymes that have increased enzymatic activity relative to a parental control enzyme.
Claims12
A variant Amorpha-4,11-diene 12-monooxygenase polypeptide comprising the amino acid sequence of SEQ ID NO:1 comprising one or more amino acid substitutions selected from A8D, E128N, R172I, V219I, T240N, K244C, F282N, L333M, Q345K, L350F, T420R, A442K, A442S, Q449K, S468C, T486C, L489C, and V491 M.
Dependencies are inferred from the claim text.