Modified glutamate dehydrogenase and the use thereof
AssigneeHunan Lier Biotech Co., Ltd
InventorsXinkai Xie, Wei Xu, Junying Fan
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Abstract
The present invention refers to a modified glutamate dehydrogenase (GluDH). In particular the modified GluDH of the present invention has an increased activity for catalyzing the reaction of 4-(hydroxymethylphosphinyl)-2-oxobutanoic acid (PPO) and an amino donor to generate L-glufosinate and/or an improved dynamic property. The present invention also refers to the polynucleotide encoding the modified GluDH of the present invention, the vector and host cell for expressing the modified GluDH of the present invention and the method of producing L-glufosinate with the modified GluDH and host cell of the present invention.
Claims16
A modified glutamate dehydrogenase (GluDH) comprising an amino acid sequence selected from one of the group consisting of SEQ ID NOs:
- 5-8, 11, 13, 16-19, 21, 27, 28, 30, 32, 34, 35, 37-48, 50, 51, and 53-72.
A modified glutamate dehydrogenase (GluDH) having a sequence identity of at least 95% to SEQ ID NO:1 or SEQ ID NO:2, wherein the modified GluDH comprises substitutions at positions 173, 175 and 182 as compared to the initial GluDH thereof, further wherein in the modified GluDH the amino acid at position 173 is substituted with G, the amino acid at position 175 is substituted with G, and the amino acid at position 182 is substituted with R, wherein the positions are numbered by reference to SEQ ID NO:
- 1, and wherein the activity of the modified GluDH for catalyzing a reaction of 2-carbonyl-4-(hydroxymethylphosphono)butyric acid (PPO) and an amino donor to generate L-glufosinate is at least 110% of the activity of SEQ ID NO:3 or SEQ ID NO:31 for catalyzing the reaction.
Dependencies are inferred from the claim text.