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US 12,735,677 B2Granted
Method for producing astrocyte-like cells
AssigneesJsr Corporation, Keio University
InventorsMitsuru Ishikawa, Hideyuki Okano
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Abstract
A method is provided for producing astrocyte-like cells, including (A) upregulating transcription factors including SRY-box transcription factor 9 (SOX9), nuclear factor IA (NFIA), and nuclear factor IB (NFIB) in human pluripotent stein cells and (B) culturing the human pluripotent stem cells, in which the transcription factors are upregulated, and consequently differentiating the human pluripotent stem cells into astrocyte-like cells.
Claims8
A method for producing astrocyte-like cells, comprising:
- (A) upregulating transcription factors including SRY-box transcription factor 9 (SOX9), nuclear factor IA (NFIA), and nuclear factor IB (NFIB) in human pluripotent stem cells;
- and
- (B) culturing the human pluripotent stem cells, in which the transcription factors are upregulated, and consequently differentiating the human pluripotent stem cells into astrocyte-like cells,
- wherein (A) upregulating is carried out by introducing a vector containing nucleic acids encoding the transcription factors into the human pluripotent stem cells,
- wherein the vector contains a nucleic acid consisting of a base sequence represented by Formula (1),
- F1—S1—F2—S2—F3 (1),
- wherein in Formula (1), F1, F2, and F3 represent, in no particular order, a base sequence encoding SOX9, a base sequence encoding NFIA, and a base sequence encoding NFIB, and S1 and S2 represent a separator base sequence,
- whereby in (B) culturing, abundances of SOX9, NFIA, and NFIB in the human pluripotent stem cells satisfy 1:1:1 in terms of molar ratio.
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