Affinity-tagged photoswitches and methods of use thereof
AssigneeThe Regents of the University of California
InventorsEhud Y. Isacoff, Dirk Trauner, Johannes Broichhagen, Joshua Levitz
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Abstract
The present disclosure provides a conjugate comprising: a) an affinity agent that specifically binds a target ligand-binding polypeptide; and b) a photoisomerizable regulator comprising: i) a photoisomerizable moiety; and ii) a ligand that binds to the target ligand-binding polypeptide. The present disclosure provides cells comprising a conjugate of the present disclosure. The present disclosure provides methods of using a conjugate of the present disclosure to modulate activity of a target polypeptide, and to modulate activity of a target cell or cell population.
Claims9
A conjugate comprising:
- a) an affinity agent that specifically binds to a metabotropic glutamate receptor, wherein the affinity agent is a nanobody comprising the sequence of SEQ ID NO:
- 4;
- b) a linker;
- and
- c) a photoisomerizable regulator comprising:
- i) a photoisomerizable group, wherein the photoisomerizable group comprises an azobenzene;
- and
- ii) a ligand that binds to the metabotropic glutamate receptor, wherein the ligand is a D stereoisomer,
- wherein the conjugate is configured to attach to the metabotropic glutamate receptor solely via binding of the affinity agent to the metabotropic glutamate receptor.
Dependencies are inferred from the claim text.