Method for mechanically separating tissue and cells constituting biological tissue

A method for mechanically separating tissue and cells, the method comprising steps of:

• preparing adipose tissue;
• diluting the adipose tissue with a diluent to prepare an adipose tissue mixed solution, wherein the preparing of the adipose tissue mixed solution includes adjusting the number of cells and cell density per unit volume of the tissue and cells recovered according to a dilution ratio of the diluent;
• micronizing the adipose tissue, and tissue and cells constituting the adipose tissue, in the adipose tissue mixed solution;
• and
• separating the adipose tissue and the tissue and cells constituting the adipose tissue, in the micronized adipose tissue mixed solution,
• wherein the micronizing of the adipose tissue and the tissue and cells constituting the adipose tissue includes moving the adipose tissue mixed solution through a plurality of screen through holes back and forth repeatedly, wherein a plurality of screens comprise sharp edges formed by a plurality of protrusions protruding toward a center of the plurality of screen through holes, and
• wherein the micronizing the adipose tissue, and tissue and cells constituting the adipose tissue includes micronizing to a size of 10 μm to 4,000 μm performed by tearing the adipose tissue as the adipose tissue mixed solution moves back and forth through the sharp edges.