Device and method for separating single colony in deep-sea in-situ environment

A device for separating a single colony in a deep-sea in-situ environment, comprising a central control system, a separation operation incubator, an environmental parameter detection unit, a pressure control unit, a temperature control unit, a liquid injection unit, and a sampling unit, wherein

• the environmental parameter detection unit comprises a temperature sensor and a pressure sensor,
• the separation operation incubator comprises a base, and a cover fixedly connected to the base;
• the cover is used as an observation area and a separation operation area, and the base is used as a culture area;
• the observation area is used for observation of separation and culture processes;
• an inner slide rail is arranged in the separation operation area according to separation operation needs, and a sampling probe device capable of sliding on the inner slide rail is installed on the inner slide rail, and used for carrying out a streaking operation and a sampling operation on microorganisms;
• a culture medium is arranged in the culture area, and used for culturing streaked microbial colonies;
• the environmental parameter detection unit is arranged in the separation operation incubator, and used for detecting temperature and pressure changes in the separation operation incubator in real time and transmitting data to the central control system;
• the pressure control unit and the temperature control unit are respectively connected to the separation operation incubator to ensure that a pressure and a temperature in the separation operation incubator are consistent with a growth and culture environment of microorganisms;
• the liquid injection unit is used for injecting an enriched microbial bacteria liquid into the separation operation incubator to achieve dipping and streaking by the sampling probe device;
• the sampling unit is used for carrying out pressure-holding sampling on microorganisms;
• a control end of the sampling probe device, a control end of the pressure control unit, a control end of the temperature control unit, a control end of the liquid injection unit, and a control end of the sampling unit are all electrically connected to the central control system,
• the observation area comprises an outer slide rail arranged on an outer side surface of the cover, a connection bracket slidably connected to the outer slide rail, an observation device fixed to an end of the connection bracket, and a visible window arranged on a surface of the cover;
• and a data output end of the observation device of the observation area is electrically connected to the central control system.