Cell culture substrate and cell culture scaffold kit

A cell culture substrate used to prepare a cell culture scaffold,

• the cell culture substrate comprising:
• a base;
• a conductive layer;
• and
• an insulating layer,
• wherein:
• the cell culture substrate has, in a plan view, a plurality of regions aligned along a first direction, the plurality of regions including a first region, a second region, and a third region sequentially arranged along the first direction,
• a surface of the cell culture substrate includes a first segment formed by the insulating layer, and a second segment formed by the conductive layer,
• the second segment has a relatively higher surface free energy than the first segment,
• a ratio of an area of the second segment to a particular area in the plan view is greater in the second region than in the first region, and greater in the third region than in the second region, and
• a ratio of an area of the first segment to the particular area in the plan view is smaller in the second region than in the first region, and smaller in the third region than in the second region.