Method for purifying Fomes officinalis polysaccharide by sequential simulated moving chromatography

A method for purifying a Fomes officinalis polysaccharide by sequential simulated moving chromatography, comprising the following steps:

• step 1, leaching Fomes officinalis to obtain a polysaccharide extractive solution;
• step 2, purifying the polysaccharide extractive solution by sequential simulated moving chromatography to obtain a purified solution;
• and
• step 3, subjecting the purified solution to a treatment of refining and removing impurities, followed by concentrating and drying to obtain the Fomes officinalis polysaccharide;
• wherein in the step 2, the sequential simulated moving chromatography comprises six chromatography columns, numbered columns 1-6;
• column 1 is configured with a feeding inlet, an eluent inlet, a discharge outlet and an impurity component outlet;
• column 2, column 3 and column 6 are configured with a feeding inlet and a discharge outlet;
• column 4 is configured with a feeding inlet, a polysaccharide raw material inlet and a discharge outlet;
• column 5 is configured with a feeding inlet, a discharge outlet and a polysaccharide component outlet;
• the discharge outlet of column 1 is connected with the feeding inlet of the column 2, the discharge outlet of column 2 is connected with the feeding inlet of the column 3, the discharge outlet of column 3 is connected with the feeding inlet of the column 4, the discharge outlet of column 4 is connected with the feeding inlet of the column 5, the discharge outlet of column 5 is connected with the feeding inlet of the column 6, and finally the discharge outlet of column 6 is connected with the feeding inlet of the column 1 to form a closed loop.