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US 12,735,486 B2Granted
Multivalent protein complexes
AssigneeGuangzhou Binding Technologies Co., Limited
InventorsYinong Zong, Yang Liu
Life of this patent
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Abstract
This disclosure is related to methods of making and using a multi-protein complex. The complex can create a multivalent binding mechanism for multiple targets in a solution or on a surface. The complex has various applications, and can be used, e.g., for activation and/or expansion of T cells.
Claims4
A method for activating or expanding T cells, the method comprising
- contacting the T cells with a soluble multi-protein complex comprising
- a polypeptide comprising two or more Fc-binding domains, wherein each of the Fc-binding domains includes an engineered IgG-binding domain of Staphylococcal Protein A and has a sequence as set forth in SEQ ID NO:
- 6, and
- two or more Fc-containing proteins including an anti-CD3 antibody and an anti-CD28 antibody, each of the Fc-containing proteins comprising an Fc region,
- wherein each Fc-containing protein binds to the Fc-binding domain in the polypeptide by noncovalent interaction.
2 more elements
A method for activating or expanding T cells, the method comprising
- contacting the T cells with
- a first soluble multi-protein complex comprising
- a first polypeptide comprising two or more Fc-binding domains, wherein each of the Fc-binding domains of the first polypeptide includes an engineered IgG-binding domain of Staphylococcal Protein A and has a sequence as set forth in SEQ ID NO:
- 6, and
- two or more anti-CD3 antibodies, wherein the anti-CD3 antibodies bind to the Fc-binding domain in the first polypeptide by noncovalent interaction, and
- a second soluble multi-protein complex comprising
- a second polypeptide comprising two or more Fc-binding domains, wherein each of the Fc-binding domains of the second polypeptide includes an engineered IgG-binding domain of Staphylococcal Protein A and has a sequence as set forth in SEQ ID NO:
- 6, and
- two or more anti-CD28 antibodies, wherein the anti-CD28 antibodies bind to the Fc-binding domain in the second polypeptide by noncovalent interaction.
6 more elements
Dependencies are inferred from the claim text.