Bispecific antigen binding molecules targeting OX40 and FAP

A bispecific antigen binding molecule, comprising

• (a) at least two antigen binding domains capable of specific binding to OX40,
• (b) an antigen binding domain capable of specific binding to Fibroblast Activation Protein (FAP) comprising a heavy chain variable region (VHFAP) comprising
• (i) CDR-H1 comprising the amino acid sequence of SEQ ID NO:3, (ii) CDR-H2 comprising the amino acid sequence selected from the group consisting of SEQ ID NO:
• 4, SEQ ID NO:11 and SEQ ID NO:12, and (iii) CDR-H3 comprising the amino acid sequence of SEQ ID NO:5, and a light chain variable region (VLFAP) comprising (iv) CDR-L1 comprising the amino acid sequence selected from the group consisting of SEQ ID NO:6, SEQ ID NO:13 and SEQ ID NO:
• 14, (v) CDR-L2 comprising the amino acid sequence of SEQ ID NO:7, and (vi) CDR-L3 comprising the amino acid sequence of SEQ ID NO:8, and
• (c) a Fc region composed of a first and a second subunit capable of stable association,
• wherein the antigen binding domains capable of specific binding to OX40 comprise:
• (i) a heavy chain variable region (VHOX40) comprising (i) CDR-H1 comprising the amino acid sequence of SEQ ID NO:43, (ii) CDR-H2 comprising the amino acid sequence of SEQ ID NO:44, and (iii) CDR-H3 comprising the amino acid sequence of SEQ ID NO:45, and a light chain variable region (VLOX40) comprising (iv) CDR-L1 comprising the amino acid sequence of SEQ ID NO:46, (v) CDR-L2 comprising the amino acid sequence of SEQ ID NO:47, and (vi) CDR-L3 comprising the amino acid sequence of SEQ ID NO:48;
• or
• (ii) a heavy chain variable region (VHOX40) comprising the amino acid sequence of SEQ ID NO:59 and a light chain variable region (VLOX40) comprising the amino acid sequence of SEQ ID NO:34, or
• (iii) a heavy chain variable region (VHOX40) comprising the amino acid sequence of SEQ ID NO:60 and a light chain variable region (VLOX40) comprising the amino acid sequence of SEQ ID NO:34, or
• (iv) a heavy chain variable region (VHOX40) comprising the amino acid sequence of SEQ ID NO:61 and a light chain variable region (VLOX40) comprising the amino acid sequence of SEQ ID NO:34.