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US 12,735,462 B2Granted
GlxR protein variant or threonine production method using same
AssigneeCj Cheiljedang Corporation
InventorsYee-Seul So, Su Yon Kwon, Kwang Woo Lee, Jihyun Shim
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Abstract
The present application relates to a GlxR protein variant or a threonine production method using same, and the GlxR protein variant according to one embodiment is introduced into a microorganism to reduce by-product output and remarkably increase threonine production capacity.
Claims20
A glyoxylate bypass regulator (GlxR) protein variant, comprising the amino acid sequence at least 95% identical to the amino acid sequence of SEQ ID NO:
- 1 and further comprising at least one mutation selected from the group consisting of (1) to (4) below:
- (1) substitution of the residue corresponding to the 45th amino acid of SEQ ID NO:
- 1 with alanine, serine, phenylalanine or lysine;
- (2) substitution of the residue corresponding to the 66th amino acid of SEQ ID NO:
- 1 with alanine, phenylalanine or lysine;
- (3) substitution of the residue corresponding to the 166th amino acid of SEQ ID NO:
- 1 with aspartic acid, phenylalanine or lysine;
- and
- (4) substitution of the residue corresponding to the 168th amino acid of SEQ ID NO:
- 1 with alanine, phenylalanine or lysine.
7 more elements
Dependencies are inferred from the claim text.