Hemostatic elastin-like polypeptides
AssigneeUniversitäT Basel
InventorsMichael Adam Nash, Ivan Urosev
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Abstract
The present invention relates to a hemostatic elastin-like polypeptide comprising a glutamine embedded in a Q-block sequence and, optionally, a lysine embedded in a K-block sequence. Under physiological setting, the Q-block sequence and the K-block sequence are recognized by human transglutaminase factor XIIIa and crosslinked with fibrin networks. The present invention also relates to the medical use of the polypeptide, to a nucleic acid sequence encoding the polypeptide, to an expression vector comprising the nucleic acid sequence, and to a cell comprising the nucleic acid sequence or the expression vector.
Claims14
A polypeptide consisting of,
- a. a transglutaminase inhibitor sequence selected from the group consisting of:
- i.
- (SEQ ID NO 3)
- DQMMLPWPAVAL,
- ii.
- (SEQ ID NO 4)
- WQHKIDLRYNGA,
- iii.
- (SEQ ID NO 5)
- SQHPLPWPVLML,
- iv.
- (SEQ ID NO 6)
- EQFPIAFPRYSI,
- v.
- (SEQ ID NO 7)
- SEQHLLKWPPWH,
- vi.
- (SEQ ID NO 8)
- WQIPVDWPPLPP,
- vii.
- (SEQ ID NO 9)
- DQWMMAWPSLTL,
- and
- viii.
- (SEQ ID NO 10)
- SQIPMAWPLLSL,
- b. a plurality of ELP sequences of the sequence VPGXG (SEQ ID NO 12), wherein each X is independently selected from the group consisting of any proteogenic amino acid except Pro, and
- c. optionally, a spacer sequence comprising at least one lysine residue wherein
- the polypeptide consists of an amino acid sequence more than 90% identical to the polypeptide sequence of SEQ ID NO 1 or SEQ ID NO 16, and
- has at least 85% biological activity of the polypeptide sequence of SEQ ID NO 1 or SEQ ID NO 16.
Dependencies are inferred from the claim text.