Bioprocess with reduced fouling on surfaces

A process comprising:

• (a) providing an aqueous solution comprising a protein and a polyalkoxy fatty acyl surfactant of formula I
• wherein R1—C(═O) is a fatty acyl group, R2 is H or a substituted or unsubstituted hydrocarbyl group, X1 is O or NH, X2 is O or NH, n is 0, 1, 2, 3, 4, or 5, R3 is a polymeric group comprising polymerized units of formula II and III
• (b) subjecting the aqueous solution to a bioprocess comprising:
• filtering the aqueous solution with a filter selected from the group consisting of a polyvinylidene fluoride (PVDF) filter, a polyethersulfone (PES) filter, a polypropylene filter, a cellulose filter, a nylon filter, and any combination thereof to produce a first filtrate, and
• subjecting the first filtrate to a chromatography column comprising a chromatography resin selected from the group consisting of sulfopropyl-functionalized cross-linked agarose, Protein A, quaternary ammonium-functionalized cross-linked agarose, a hydrophobic interaction chromatography resin, and any combinations thereof to produce a second filtrate,
• wherein the filter exhibits reduced fouling by the protein as compared to a filter subjected to the bioprocess with the aqueous solution having polysorbate 80 in place of the polyalkoxy fatty acyl surfactant.