Allogeneic implants for the treatment of cartilage injuries

An implant or construct for the treatment of cartilage lesions in joints of a human subject comprising:

• a) a population of cadaveric P0 chondrocytes isolated from a cartilage of a cadaveric young human donor having an age range of 14 to 20 years,
• b) the chondrocytes are seeded onto a hyaluronic acid scaffold,
• c) the seeded chondrocytes are sealed with a fibrin glue, wherein the sealed chondrocytes are cultured in a culture medium containing an autologous serum for a period of 5 to 7 days prior to use;
• d) wherein the cadaveric P0 chondrocytes have been cryopreserved;
• and
• e) wherein the chondrocytes of the implant induce the formation of a hyaline cartilage having a modified O'Driscoll histological score of 9.57±1.27 (mean SD), with the formation of isogenic groups and an extracellular matrix displaying markers characteristic of a hyaline cartilage.