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US 12,734,274 B2Granted
Methods for preparation of a terminally sterilized hydrogel or colloidal suspension derived from extracellular matrix, and uses thereof
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsStephen Francis Badylak, George S. Hussey
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Abstract
A method is disclosed for treating a fistula in a subject, such as, but not limited to, an anal fistula. In some embodiments, the method includes administering locally to the fistula in the subject an effective amount of a composition comprising a mammalian acoustic extracellular matrix (ECM) hydrogel, and optionally trehalose. Compositions of use in these methods are also disclosed.
Claims21
A method of treating a fistula in a subject, comprising:
- administering locally to the fistula in the subject an effective amount of a composition comprising a mammalian extracellular matrix (ECM) hydrogel, wherein:
- a) the mammalian ECM hydrogel is thermoreversible, wherein the mammalian ECM hydrogel is in a gel phase at temperatures below about 37° C. and transitions to a liquid phase at temperatures above about 37° C.,
- b) the mammalian ECM hydrogel comprises solubilized ECM at a concentration of about 0.1 mg/mL to about 1,000 mg/mL, and
- c) the composition has a storage modulus (G′) to loss modulus (G″) ratio in the range of about 6:1 to about 12:1 at 37° C.,
- thereby treating the fistula in the subject.
2 more elements
A composition comprising:
- i) a mammalian extracellular matrix (ECM) hydrogel, wherein:
- a) the mammalian ECM hydrogel is thermoreversible, wherein the mammalian ECM hydrogel is in a gel phase at temperatures below about 37° C. and transitions to a liquid phase at temperatures above about 37° C.,
- b) the mammalian ECM hydrogel comprises solubilized ECM at a concentration of about 0.1 mg/mL to about 1,000 mg/mL, and
- c) the composition has a storage modulus (G′) to loss modulus (G″) ratio of about 6:1 to about 12:1 at 37° C.;
- ii) 0.1 mg/mL to about 700 mg/mL trehalose;
- and
- iii) about 1% to about 30% weight per volume of comminuted ECM that is not solubilized in the hydrogel.
4 more elements
Dependencies are inferred from the claim text.