Processes of preparing mRNA-loaded lipid nanoparticles
AssigneeTranslate Bio, Inc.
InventorsShrirang Karve, Ashish Sarode, Frank DeRosa
Life of this patent
Estimating the term…
Abstract
The present invention provides an improved process for lipid nanoparticle formulation and mRNA encapsulation. In some embodiments, the present invention provides a process of encapsulating messenger RNA (mRNA) in lipid nanoparticles comprising a step of mixing a suspension of preformed lipid nanoparticles and mRNA.
Claims18
A composition comprising a first set of mRNA-encapsulating lipid nanoparticles (LNPs) comprising a first cationic lipid, a first non-cationic lipid, and a first PEG-modified lipid and a second set of empty LNPs comprising a second cationic lipid, a second non-cationic lipid, and a second PEG-modified lipid, wherein the first set of LNPs and the second set of LNPs are present at a ratio ranging from 20:1 to 1:20, wherein the first set of LNPs and the second set of LNPs have different lipid formulations.
Dependencies are inferred from the claim text.