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AssigneeUniversity of Virginia Patent Foundation
InventorsSteven L. Zeichner, Barbie Ganser-Pornillos, Owen Pornillos
Estimating the term…
Disclosed herein are bacteria-based HIV MPER vaccine candidates, as well as bacteria-based candidates for other viruses and for bacteria. The HIV vaccine candidates express MPER-derived antigens on their surfaces using Gram autotransporters. The surface-expressed MPER antigens bind several different MPER-directed anti-HIV Broadly Neutralizing Monoclonal Antibodies. When the bacteria expressing the MPER-derived antigens on their surfaces are used to immunize mice they elicit the production of sera and vaginal wash material that bind the bacteria expressing the MPER antigens. At least one of the bacteria expressing MPER-derived antigens on their surfaces elicits the production of sera with anti-HIV neutralizing activity. Killed whole cell and live Salmonella expressing the MPER derived antigens on their surfaces constitute new approaches to HIV vaccine develop that is plausible and that could ultimately yield an inexpensive, globally appropriate candidate vaccine that could be rapidly produced and deployed largely using currently available technology.
A modified bacterium, or a derivative thereof, comprising a heterologous antigen expressed on a surface of a membrane of the bacterium, wherein the heterologous antigen is heterologous to the modified bacterium, wherein the heterologous antigen comprises a membrane-proximal external region (MPER) peptide, wherein the MPER peptide comprises a sequence selected from the group consisting of SEQ ID NOs:
An expression vector comprising a nucleotide sequence encoding an antigen, wherein the expression vector is configured to express the antigen on a surface of a bacterium modified to include the expression vector, wherein the expression vector comprises a monomeric or trimeric autotransporter (AT) expression vector, and wherein the antigen comprises a membrane-proximal external region (MPER) peptide, wherein the MPER peptide comprises a sequence selected from the group consisting of SEQ ID NOs:
Dependencies are inferred from the claim text.