3D printing of biomimetic flexible multilayer blood vessels
AssigneeThe George Washington University
InventorsSung Yun Hann, Haitao Cui, Lijie Grace Zhang
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Abstract
Systems and methods for producing artificial blood vessels. In certain embodiments, the method for producing blood vessels includes printing an elastic outer layer and removing polyvinyl alcohol component from the elastic outer layer. The process then involves forming a first inner layer of smooth muscle cells, wherein the smooth muscle cells are mixed with 5 fibrinogen solution and extruded with thrombin to form a smooth muscle cell gel, and forming a second inner layer of endothelial cells, wherein the endothelial cells are mixed with fibrinogen solution and extruded with thrombin to form an endothelial cell gel.
Claims17
A method for producing a blood vessel comprising:
- printing an outer layer;
- removing a polyvinyl alcohol component from the outer layer to produce an elastic outer layer;
- forming a first inner layer comprised of smooth muscle cells comprised of smooth muscle cell gel comprised of smooth muscle cells, fibrinogen, and thrombin, wherein the smooth muscle cells are first mixed with a fibrinogen solution and then coaxially extruded with the thrombin;
- and
- forming a second inner layer comprised of an endothelial cell gel comprised of endothelial cells, a fibrinogen, and thrombin, wherein the first inner layer is between the elastic outer layer and the second inner layer.
A method for producing a blood vessel comprising:
- printing an outer layer;
- removing a polyvinyl alcohol component from the outer layer to produce an elastic outer layer;
- forming a first inner layer comprised of smooth muscle cells comprised of smooth muscle cell gel comprised of smooth muscle cells, fibrinogen, and thrombin;
- and
- forming a second inner layer comprised of an endothelial cell gel comprised of endothelial cells, fibrinogen, and thrombin, wherein the endothelial cells are first mixed with a fibrinogen solution and then coaxially extruded with the thrombin, and wherein the first inner layer is between the elastic outer layer and the second inner layer.
Dependencies are inferred from the claim text.