Fluid control devices and methods of using the same

A fluid control device, comprising:

• a housing having an inlet configured to establish fluid communication with a bodily fluid source and an outlet configured to be coupled to a fluid collection device, the housing defining a sequestration flow path in fluid communication with the inlet and a sampling flow path in fluid communication with the outlet;
• a first flow controller having a flow state and a non-flow state, the first flow controller in the flow state configured to facilitate, in response to a negative pressure differential between the inlet and the outlet, a flow of a bodily fluid into the sequestration flow path and a flow of a gas from the sequestration flow path, through the first flow controller, and to the outlet, the first flow controller in the non-flow state configured to sequester the sequestration flow path from the outlet;
• and
• a second flow controller having a diaphragm that defines a portion of the sequestration flow path, the diaphragm configured to transition from a first configuration to a second configuration in response to an increase in a portion of the negative pressure differential between the inlet and the outlet that is applied across the diaphragm when the first flow controller is in the non-flow state, the second flow controller further having a barrier configured to be transitioned, at least in part by the diaphragm due to the diaphragm transitioning, from a first state in which the barrier separates at least a portion of the sampling flow path from the inlet to a second state in which the sampling flow path fluidically couples the inlet to the outlet.