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US 12,733,846 B2Granted
Analyte sensors with metal-containing redox mediators and methods of using the same
AssigneeAbbott Diabetes Care Inc.
InventorsJohn V. LaTour, Kevin P. Wallis, Phu Le, Udo Hoss
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Abstract
The present disclosure provides redox mediators having two tridentate ligands and analyte sensors comprising such redox mediators. The present disclosure further provides methods of using such analyte sensors for detecting one or more analytes present in a biological sample of a subject.
Claims34
An analyte sensor comprising:
- (i) a distal end comprising at least a first working electrode;
- (ii) a first active area disposed upon a surface of the first working electrode and responsive to a first analyte, wherein the first active area comprises a first polymer, a first redox mediator covalently bonded to the first polymer, and at least one enzyme responsive to the first analyte;
- wherein the first redox mediator has a structure of:
- wherein M is iron, ruthenium, osmium, cobalt, or vanadium;
- wherein n is I, II, III, IV or V;
- wherein R1, R3, R′1, and R′3 are each independently a C1-C12 straight or branched chain alkyl group;
- wherein R2 is selected from the group consisting of H and an electron donating group;
- wherein R′2 is a linking group that covalently bonds the first redox mediator to the first polymer;
- and
- (iii) a mass transport limiting membrane permeable to the first analyte that overcoats at least the first active area.
7 more elements
A method comprising:
- (i) providing an analyte sensor comprising:
- (a) a distal end comprising at least a first working electrode;
- (b) a first active area disposed upon a surface of the first working electrode and responsive to the first analyte, wherein the first active area comprises a first polymer, a first redox mediator covalently bonded to the first polymer, and at least one enzyme responsive to the first analyte;
- wherein the first redox mediator has a structure of
- wherein M is iron, ruthenium, osmium, cobalt, or vanadium;
- wherein n is I, II, III, IV, or V;
- wherein R1, R3, R′1, and R′3 are each independently a C1-C12 straight or branched chain alkyl group;
- wherein R2 is selected from the group consisting of H and an electron donating group;
- wherein R′2 is a linking group that covalently bonds the first redox mediator to the first polymer;
- and
- (c) a mass transport limiting membrane permeable to the first analyte that overcoats at least the first active area;
- (ii) applying a potential to the first working electrode;
- (iii) obtaining a first signal at or above an oxidation-reduction potential of the first active area, the first signal being proportional to a concentration of a first analyte in a fluid contacting the first active area;
- and
- (iv) correlating the first signal to the concentration of the first analyte in the fluid.
12 more elements
An analyte sensor comprising:
- (i) a distal end comprising at least a first working electrode;
- (ii) a first active area disposed upon a surface of the first working electrode and responsive to a first analyte, wherein the first active area comprises a first polymer, a first redox mediator covalently bonded to the first polymer, and at least one enzyme responsive to the first analyte;
- wherein the first redox mediator has a structure of:
- wherein n is I, II, III, IV or V;
- wherein R2 is selected from the group consisting of H and an electron donating group;
- wherein R′2 is a linking group that covalently bonds the first redox mediator to the first polymer;
- and
- (iii) a mass transport limiting membrane permeable to the first analyte that overcoats at least the first active area.
5 more elements
Dependencies are inferred from the claim text.