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US 12,733,662 B2Granted
Millet and food products with reduced lipase activity, genes and implementation thereof
OwnerRasika Rajendra Aher, Pooja Bhatnagar-Mathur +6inventors
InventorsRasika Rajendra Aher, Pooja Bhatnagar-Mathur, John D. Everard, Kayla S. Flyckt, William James Gordon-Kamm, Sudhakar Reddy Palakolanu, Kiran K. Sharma, Laura L. Wayne
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Abstract
Provided are millet and food products made from millet having reduced lipase activity. Also provided are nucleic acid sequences, constructs, expression cadettes, markers, primers probes, methods, gene editing tools, and materials useful in producing millet with reduced lipase activity as well as millet food products with reduced rancidity.
Claims4
A method of modifying a millet plant, tissue, or cell thereof comprising:
- a) providing a pearl millet plant tissue, or cell thereof comprising a genomic sequence that encodes a triacylglycerol (TAG) lipase polypeptide;
- and
- b) modifying the genomic sequence encoding the TAG lipase to encode a modified TAG lipase, wherein the modified TAG lipase
- has a substitution or is truncated at the amino acid position corresponding to (i) position 12, 18, 19, 20, 23, 26, 37, 38, 42, 43, 44, 96, 97, 98, 114, 131, 135, 144, 145, 146, 167, 168, 169, 172, 205, 206, 215, 221,222, 237, 246, 263, 289, 290, 298, 299, 330, 339, or 346 of SEQ ID NO:2 or (ii) position 220, 223, 265, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, or 332 of SEQ ID NO:4, or
- encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:7, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:
- 21, SEQ ID NO:25, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:22, SEQ ID NO:
- 26, SEQ ID NO:28, or SEQ ID NO:30,
- to thereby produce a modified pearl millet plant tissue, or cell thereof.
5 more elements
Dependencies are inferred from the claim text.