Display substrate and method of manufacturing display substrate

A method of manufacturing a display substrate, the display substrate comprising a base substrate and a plurality of sub-pixels disposed on the base substrate, wherein the sub-pixel comprises:

• a light-emitting element having a first electrode, a second electrode, and a light-emitting layer located between the first electrode and the second electrode, wherein the first electrode comprises a transparent material layer;
• and a pixel defining layer located on a side of the first electrode away from the base substrate, wherein the pixel defining layer has an opening, an edge of the pixel defining layer has an undercut structure, a center of the transparent material layer is exposed from the opening, and an edge of the transparent material layer is covered by the pixel defining layer, wherein the pixel defining layer comprises a first pixel defining sub-layer and a second pixel defining sub-layer, wherein the second pixel defining sub-layer covers the first pixel defining sub-layer and forms the undercut structure with the first pixel defining sub-layer, and a thickness of the first pixel defining sub-layer is greater than a thickness of the second pixel defining sub-layer;
• and wherein the pixel defining layer comprises a first region close to the undercut structure and a second region connected to the first region and away from the undercut structure, with a thickness of the first region greater than a thickness of the second region;
• wherein the method comprising:
• for each sub-pixel,
• forming, on the base structure, the first electrode of the light-emitting element of the sub-pixel, the light-emitting layer of the light-emitting element of the sub-pixel, the pixel defining layer, and the second electrode of the light-emitting element of the sub-pixel in sequence.