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US 12,730,292 B2Granted
Transverse sheet illumination microscopy(TranSIM)
AssigneeThe Regents of the University of California
InventorsKatsushi Arisaka, Laurent A. Bentolila, Javier Carmona, Jr., Blake A. Madruga
Life of this patent
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Abstract
Methods and apparatus for transverse sheet illuminated multiple plane imaging that can achieve simultaneous imaging of multiple z-planes in a laser scanning confocal fluorescence microscope.
Claims11
A transverse sheet illumination microscopy apparatus, comprising:
- (a) an illumination unit configured to generate multiplexed beams of light for illuminating a sample;
- (b) wherein the illumination unit is configured to illuminate multiple planes by spatially separating multiple beams in depth (Z) and laterally (Y), whereby the planes can be separated;
- (c) an imaging unit configured to image fluorescence from a sample in response to illumination of the sample by said beams of light;
- (d) the imaging unit comprising a linear reflection cavity with an imaging sensor;
- (e) wherein the imaging unit is configured to select one plane at a time from a plurality of image planes from the sample and remap the selected plane to the imaging sensor;
- and
- (f) wherein each round trip in the linear reflection cavity allows for an additional plane to be segmented and re-adjusted onto the imaging sensor for imaging the sample.
4 more elements
Dependencies are inferred from the claim text.