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US 12,730,116 B2Granted
Method for reducing measurement error
AssigneeSekisui Medical Co., Ltd.
InventorsSatoshi Machida, Hiroki Osako, Kouji Yamamoto, Yuuri Hayakawa
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AssigneeSekisui Medical Co., Ltd.
InventorsSatoshi Machida, Hiroki Osako, Kouji Yamamoto, Yuuri Hayakawa
Estimating the term…
An object of the present invention is to provide a method for measuring an object to be measured in a specimen by an enzymatic method, the measurement method being able to suppress the positive influence of peroxide derived from the specimen. More specifically, an object of the present invention is to provide a measurement method and a measurement reagent that can suppress elevation in value regardless of whether or not the specimen is a catalase-free specimen. Provided is a measurement method that can accurately quantify hydrogen peroxide derived from an object to be measured, without influence derived from a specimen, by contacting the specimen with an enzyme in the presence of at least one compound selected from the group consisting of a compound represented by the following general formula (I), a benzimidazole derivative having an electron-donating substituent at position 2, and histidine, wherein R1 and R2 are the same or different and each represent hydrogen, a linear or branched alkyl group having 1 to 6 carbon atoms and optionally having a substituent, an aryl group optionally having a substituent, or an alkyloxy group having 1 to 6 carbon atoms.
A method for measuring a component to be measured by quantifying hydrogen peroxide produced through a reaction of the component to be measured in a specimen with an enzyme, the measurement method comprising:
A measurement reagent comprising an enzyme that acts on a component to be measured to produce hydrogen peroxide, a coloring agent, peroxidase and (I) phenylglyoxal;
A measurement reagent kit comprising an enzyme that acts on a component to be measured to produce hydrogen peroxide, a coloring agent, peroxidase and phenylglyoxal,
A method for reducing a measurement error in a method for measuring a component to be measured by circumventing an influence of hydrogen peroxide derived from a component other than the component to be measured contained in a specimen, and quantifying hydrogen peroxide derived from the component to be measured by an enzymatic method, comprising:
Dependencies are inferred from the claim text.