Ribosomal protein judgement method, biological species identification method, and mass spectrometry apparatus
AssigneeShimadzu Corporation
InventorKanae Teramoto
Life of this patent
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Abstract
Provided is a ribosomal protein judgement method, comprising: an attribution step that involves comparing observed m/z values indicated by peaks on a mass spectrum detected by mass spectrometry of a sample containing ribosomal proteins, with first calculated m/z values based on a database, and attributing at least some of the peaks thus detected to ribosomal proteins; and a presumption step that involves presuming a protein corresponding to a peak with a relative intensity from 50 to 150% relative to an approximate curve or an approximate straight line plotted with relative intensities of the peaks attributed to ribosomal proteins, to be a ribosomal protein.
Claims13
A ribosomal protein determination method, comprising:
- an attribution step of comparing observed m/z values of peaks in a mass spectrum obtained by mass spectrometry of a sample containing ribosomal proteins with first calculated m/z values of ribosomal proteins based on a database, and attributing, among the obtained peaks, one or more peaks having observed m/z values that match the first calculated m/z values as ribosomal proteins peaks;
- a step of generating, from m/z values and relative intensities of the peaks attributed as ribosomal protein peaks, an approximate curve or an approximate straight line representing relative intensity as a function of m/z;
- and
- an estimation step of estimating, as a ribosomal protein peak, an unassigned peak that was not attributed as a ribosomal protein peak in the attribution step and that has a relative intensity of 50% to 150% of the relative intensity indicated by the approximate curve or the approximate straight line at the m/z value of the unassigned peak.
A mass spectrometry apparatus comprising:
- a mass separation member that separates ions based on m/z values;
- a detection member that detects the ions separated by the mass separation member;
- based on the ions detected by the detection member;
- and
- a determination member that determines, from the mass spectrum, peaks that are attributable as ribosomal protein peaks based on a database, generates, from relative intensities of the peaks attributed as ribosomal protein peaks, an approximate curve or an approximate straight line representing relative intensity as a function of m/z, and selects an unassigned peak that was not attributed as a ribosomal protein peak by the determination member and that has a relative intensity of 50% to 150% of the relative intensity indicated by the approximate curve or the approximate straight line at the m/z value of the unassigned peak.
Dependencies are inferred from the claim text.