Nano-coulter counter for detection of biological nanoparticles

A nano-Coulter counter device comprising:

• an inlet microchannel;
• a plurality of nanochannels fluidly coupled to the inlet microchannel having a first cross-dimension, each nanochannel having a nanopore with a second cross-dimension that is narrower than the first cross-dimension, wherein each nanochannel includes an inlet tapered region coupled to an inlet of the nanopore that tapers from the first cross-dimension to the second cross-dimension, wherein each nanopore has the second cross-dimension of at least about 50 nm to about 300 nm, and a pore length of at least about 50 nm to about 300 nm, wherein each nanochannel includes an outlet expansion region coupled to an outlet of the nanopore that expands from the second cross-dimension to a third cross-dimension that is larger than the second cross-dimension;
• an outlet microchannel fluidly coupled to an outlet of each of the plurality of nanochannels;
• an electrode pair having an electrode at the inlet microchannel and another electrode at the outlet microchannel;
• a power source electrically coupled with the electrode pair;
• and
• a pump operably coupled with the plurality of nanochannels.