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US 12,730,040 B2Granted
Chelator based eluents for solid phase extraction of phosphorylated compounds
AssigneeWaters Technologies Corporation
InventorsXiaoxiao Liu, Matthew Lauber, Michael Donegan
Life of this patent
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Abstract
The present technology relates to a method for improved recovery of phosphorylated compounds (e.g., phosphorylated glycans). In particular, the present technology utilizes certain chelator additives in a solution to wash and elute the phosphorylated compounds from a solid phase extraction cartridge.
Claims7
A method of performing solid phase extraction comprising:
- injecting a sample comprising one or more oligonucleotides into a solid phase extraction cartridge comprising a stationary phase, wherein the oligonucleotide is retained by the stationary phase;
- and
- washing the solid phase extraction cartridge with a divalent or higher valency carboxylic acid at a concentration of between about 10 mM to about 1 M to elute the one or more oligonucleotides from the solid phase extraction cartridge.
A method of performing solid phase extraction comprising:
- injecting a sample comprising one or more oligonucleotides into a solid phase extraction cartridge comprising a stationary phase, wherein the one or more oligonucleotides are retained by the stationary phase;
- and
- washing the solid phase extraction cartridge with an eluent solution to elute the one or more oligonucleotides from the solid phase extraction cartridge, wherein the eluent solution comprises (i) a salt to adjust ionic strength of the eluent solution and (ii) a divalent or higher valency carboxylic acid at a concentration of between about 10 mM to about 1 M.
Dependencies are inferred from the claim text.