Randomized configuration targeted integration of nucleic acids
AssigneeGenentech, Inc.
InventorsShahram Misaghi, Bradley R. Snedecor
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Abstract
The presently disclosed subject matter relates to “Randomized Configuration Targeted Integration” (also referred to herein as “Randomized Chain Targeted Integration”) (RCTI) strategies for the generation and identification of host cells capable of expressing recombinant proteins, e.g., monoclonal antibodies, as well as compositions derived from the same, e.g., bispecific antibodies, and other complex format proteins, e.g., membrane protein complexes and other difficult to express molecules.
Claims7
A method of preparing a targeted integration (TI) host cell expressing one or more sequences of interest (SOIs) comprising:
- a) providing a plurality of TI host cells;
- b) contacting the plurality of TI host cells with a plurality of vectors, wherein the vectors comprise one or more SOIs;
- c) introducing the one or more SOIs into one or more of the plurality of TI host cells;
- and
- d) selecting the TI host cells expressing the one or more SOIs;
- wherein the one or more SOIs are introduced into one or more of the plurality of TI host cells at one or more loci at least about 90% homologous to a sequence selected from:
- SEQ ID Nos. 1-12;
- NW_006874047.1;
- NW_006884592.1;
- NW_006881296.1;
- NW_003616412.1;
- NW_003615063.1;
- NW_006882936.1;
- and NW_003615411.1.
Dependencies are inferred from the claim text.