Agent interaction effects determination

A method for determining agent interaction effects on a cell population, the method comprising:

• adding a cell culture substrate into a culture container comprising N agent reservoirs at predefined positions relative to each other, wherein each agent reservoir of the N agent reservoirs comprises an agent, the N agent reservoirs enclose combination areas and N is an integer equal to or larger than three;
• placing a cell population on and/or in the cell culture substrate and culturing the cell population on and/or in the cell culture substrate for a predefined period of time while the agents in the N agent reservoirs diffuse through the cell culture substrate and form at least partly overlapping agent concentration gradients in the cell culture substrate within the combination areas and substantially non-overlapping agent concentration gradients in the cell culture substrate peripheral to an outer boundary of the N agent reservoirs;
• determining, for each agent reservoir of at least two adjacent agent reservoirs of the N agent reservoirs, an inhibition end point of an inhibition zone substantially lacking any growth of the cell population, the inhibition end point being positioned peripherally to the outer boundary of the agent reservoir;
• determining, for the at least two adjacent agent reservoirs, a growth end point of a growth zone comprising growth of the cell population within a combination area with at least partly overlapping agent concentration gradients of the agents contained in the at least two adjacent agent reservoirs;
• and
• determining an agent interaction effect between the agents contained in the at least two adjacent agent reservoirs based on the inhibition end points and the growth end point.