Fermentation process for preparing postbiotic with by-product of wheat processing

A fermentation process for preparing a postbiotic solution comprising the following steps:

• (1) preparing a fermentation matrix by mixing wheat starch wastewater and wheat bran in a mass ratio of 1:1 to 12:1 of the wheat starch wastewater to the wheat bran to obtain a resulting mixture, sterilizing the resulting mixture by heating to obtain a sterilized mixture, and cooling the sterilized mixture to room temperature to obtain a fermentation matrix;
• (2) inoculating fermentation strains by:
• (a) culturing Paenibacillus polymyxa (P. polymyxa),
• (b) culturing Brachybacterium paraconglomeratum (B. paraconglomeratum), and
• (c) culturing Flavobacterium pectinovorum (F. pectinovorum),
• mixing each resulting culture of (a)-(c) to obtain a mixed fermentation broth, inoculating the mixed fermentation broth into the fermentation matrix obtained in step (1) under sterile conditions, and conducting fermentation at 25° C. to 37° C. for 48 hours to 192 hours to produce a fermentation system;
• and
• (3) isolating and extracting the postbiotic solution by filtering the fermentation system obtained in step (2) to obtain a resulting filtrate, subjecting the resulting filtrate to primary centrifugation to obtain a first supernatant, collecting the first supernatant, and subjecting the first supernatant to secondary centrifugation to obtain a second supernatant;
• wherein the second supernatant is the postbiotic solution and
• wherein the postbiotic solution comprises a lipoteichoic acid (LTA) and a short-chain fatty acid (SCFA).