Back to results
US 12,729,387 B2Granted
Process for making flavor and fragrant compounds
AssigneeFirmenich SA
InventorsFredi Bruhlmann, Rebecca Buller
Life of this patent
Estimating the term…
Abstract
Described herein are methods of producing hexenols including: a) contacting a hydroperoxide of a polyunsaturated fatty acid with a modified hydroperoxide lyase to form a hexenal; and b) reducing the hexenal to a hexenol in the presence of a hydride donor, a ketoreductase, and a co-factor, where the contacting and reducing steps are carried out at essentially the same time in the substantial absence of baker's yeast.
Claims5
A composition comprising (Z,Z)-3,6-nonadienol, wherein the composition is produced by a method comprising:
- a) contacting a 9-hydroperoxide of a polyunsaturated fatty acid selected from the group consisting of linoleic acid and alpha linolenic acid with a 9-hydroperoxide lyase to form an aldehyde selected from the group consisting of a nonenal and nonadienal;
- and
- b) reducing the aldehyde in the presence of a hydride donor, a ketoreductase, and a co-factor;
- wherein the contacting and reducing steps are carried out concomitantly;
- wherein
- the hydride donor is selected from the group consisting of a secondary alcohol, a primary alcohol, an alkandiol and a hydroxy acid or one of its esters;
- the cofactor is selected from the group consisting of NADH and NADPH;
- the hydroperoxide lyase is stable in the presence of the hydride donor, thus allowing for the concomitant hydroperoxide cleavage and aldehyde reduction;
- and
- the method is performed in the absence of intact cells;
- wherein the composition comprises (Z,Z)-3,6-nonadienol in an amount greater than or equal to 0.74 g/l, and wherein the composition may comprise less than or equal to 1 g/l (Z)-3-nonenol.
8 more elements
A composition comprising an alcohol, wherein the composition is produced by a method comprising:
- a) contacting a 9- or 13-hydroperoxide of a polyunsaturated fatty acid selected from the group consisting of linoleic acid and alpha linolenic acid with the respective hydroperoxide lyase to form an aldehyde;
- and
- b) reducing the aldehyde to form the alcohol in the presence of a hydride donor, a ketoreductase, and a co-factor wherein the contacting and reducing steps are carried out concomitantly;
- wherein
- the hydride donor is selected from the group consisting of a secondary alcohol, a primary alcohol, an alkandiol and a hydroxy acid or one of its esters;
- the cofactor is selected from the group consisting of NADH and NADPH;
- the hydroperoxide lyase is stable in the presence of the hydride donor, thus allowing for the concomitant hydroperoxide cleavage and aldehyde reduction;
- and
- the method is performed in the absence of intact cells.
6 more elements
Dependencies are inferred from the claim text.