Alpha-amylase variants

An alpha-amylase variant of a parent alpha-amylase comprising

• a) a deletion at two or three or four positions corresponding to positions R181, G182, H183 and G184, and
• b) a set of substitutions selected from the group consisting of:
• K35A+A51T+N54A+G109A+R118T+W140Y+R172S+N174*+R181Q+E190P+I206L+I235L+Y243F+F267Y+Q280N+K281F+N299A+K302T+R320K+S334T+A339S+E345Q+E346P+V4101;
• N311K+I312L+F313L+Q319S+R320K+H324K+S334T+E345Q+E346P+L355F+T356I+Q361S+Y371M+R383K+S384H+D387E+E391K+Q394K+K395Q+P400A+V410I+E471T+D476K;
• N22Q+N25R+N28Q+S29N+A51T+N54A+N70H+R82K+S83G+Q84E+V89I+T90K+K93H+Q98N+G109A+M116D+R118T+E130V+T136L+E138K+W140Y+R142H+D144H+N150S+H152Y+S154D+R158Y+R172S+N174*+R181Q+E190P+I206L+Y243F+F267Y+Q280N+K281F+N299A+K302N+R320K+S334T+A339S+E345Q+E346P+T356I+Q361S+V410I+E471T+D476K
• H1*+N3A+M9L+K35A+W48Y+A51T+N54A+M105I+G109A+R118Q+W140Y+R172S+N174*+R181Q+E190P+I206L+V214I+I235L+Y243F+T246L+F267Y+Q280N+K281F+N299A+K302T+R320K+S334T+A339S+E345Q+E346P+V379I+M382L+V410I+D476K+K485R;
• H1A+N3A+M9L+K35A+W48Y+A51T+N54A+M105I+G109A+R118T+W140Y+R172S+N174*+R181Q+E190P+I206L+I235L+Y243F+T246L+F267Y+Q280N+K281F+N299A+K302T+R320K+S334T+A339S+E345Q+E346P+V379I+M382L+V410I+D476K;
• A51T+N54A+G109A+R118T+E134D+W140Y+R172S+E190P+I206L+Y243F+F267Y+Q280N+K281F+N299A+K302N+R320K+S334T+A339S+V410I+E471T+D476K;
• N22Q+N25K+N28Q+S29N+K35A+A51T+N54A+N70H+Q84D+V89I+T90K+K93H+Q98N+G109A+M116D+R118Q+E130V+V131I+T136L+E138K+W167F+R172S+R181Q+I206L+G229Q+I235L+Y243F+F267Y+Q280N+K281F+N299A+K302N+Q319S+R320K+S334T+A339S+F343T+E345Q+E346T+I374T+V379I+M382L+R383K+P400A+V410I+K423N+L444A+K463V+E471T+D476K;
• H1A+N3A+A51T+N54A+G109A+R118T+E134D+W140Y+R172S+N174S+E190P+I206L+Y243F+F267Y+Q280N+K281F+N299A+K302N+R320K+S334T+A339S+V4101+1411V+K423N+S437A+A442V+L444R+K445Q+Y452H+D460E+T461P+K463V+G465N+D467E+D476K+K485R;
• H1*+N3A+A51T+N54A+G109A+R118T+E134D+W140Y+R172S+N174S+E190P+I206L+Y243F+F267Y+Q280N+K281F+N299A+K302N+R320K+S334T+A339S+V4101+1411V+K423N+S437A+A442V+L444R+K445Q+Y452H+D460E+T461P+K463V+G465N+D467E+D476K+K485R;
• H1A+N3A+K35A+A51T+N54A+G109A+R118T+W140Y+R172S+N174S+R181Q+E190P+I206L+I235L+Y243F+F267Y+Q280N+K281F+N299A+K302T+R320K+S334T+A339S+E345Q+E346P+P408H+V4101+1411V+K423N+S437A+A442V+L444R+K445Q+Y452H+D460E+T461P+K463V+G465N+D467E+D476K+K485R;
• and
• H1A+H2*+N3A+M9L+K35A+W48Y+A51T+N54A+M105I+G109A+R118T+W140Y+R172S+R181Q+E188P+I204L+I233L+Y241F+T244L+F265Y+Q278N+K279F+N297A+K300T+R318K+S332T+A337S+E343Q+E344P+V377I+M380L+V408I+D474K;
• using SEQ ID NO:
• 1 for numbering;
• and wherein said variant has at least 80% but less than 100% sequence identity to the amino acid sequence to the polypeptide of SEQ ID NO:
• 1;
• wherein said variant has alpha-amylase activity;
• and wherein said variant has an Improvement Factor (IF)>1.0 for a measure of wash performance when compared to said alpha-amylase having alpha-amylase activity and comprising the polypeptide of SEQ ID NO:
• 2.