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US 12,729,374 B2Granted
Glycosyltransferase and Steviol glucoside preparation method using same
AssigneeSamyang Corporation
InventorsJeong Min Kim, Hyeok-Jin Ko, Jun-Ho Moon, Eunsoo Choi
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Abstract
The present invention discloses a glycosyltransferase and a composition and method for producing steviol glycoside using the same, and more particularly, a glycosyltransferase of rebaudioside that transfers glucose to steviol glycoside, a recombinant strain expressing the enzyme, and a method of producing rebaudioside, a steviol glycoside, using the same.
Claims7
A method for producing rebaudioside D and rebaudioside E from mixed substrates comprising rebaudioside A and stevioside comprising
- reacting, in the presence of glucose donor, at least one selected from the group consisting of an enzyme protein, a recombinant microorganism expressing the enzyme protein, cells of the microorganism, cell lysates of the microorganism, cultures of the microorganism, and extracts thereof, with the mixed substrates comprising rebaudioside A and stevioside,
- wherein the enzyme protein comprises an amino acid sequence having at least 92% of sequence identity with SEQ ID NO:
- 1 and has uridine disphosphate-glycosyltransferase (UDP-glucoslyltransferase) activity of transferring glucose to rebaudioside A and stevioside.
Dependencies are inferred from the claim text.