Porcine stomach mucosal tissue decellularization method for culturing stomach or stomach cancer tissues, composition for producing scaffold, and method for culturing tissue

A method for decellularization of porcine stomach mucosal tissue comprising:

• a chopping step of chopping porcine stomach mucosal tissue;
• a first treatment step of immersing and maintaining the chopped porcine stomach mucosal tissue in a solution containing 0.5 to 2% (w/v) sodium dodecyl sulfate and 10 to 100 mM ethylenediaminetetraacetic acid for 12 to 48 hours;
• a second treatment step of immersing and maintaining the stomach mucosal tissue, subjected to the first treatment step, in a solution containing 0.5 to 2% (w/v) polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether and 10 to 100 mM ethylenediaminetetraacetic acid for 12 to 48 hours;
• a washing step of washing the stomach mucosal tissue subjected to the second treatment step;
• and
• a third treatment step of immersing and maintaining the stomach mucosal tissue, subjected to the washing step, in a solution containing 0.05 to 0.2% (w/v) peracetic acid and 2 to 8% (v/v) ethanol for 1 to 4 hours;
• wherein the porcine stomach mucosal tissue is tissue of a mucosa layer of a porcine stomach.