Capillary-driven perfusion systems and methods of use

A perfusion system for a well plate, comprising:

• a chamber insert and a reservoir lid;
• wherein when coupled with a well plate, the chamber insert inserts into wells of the well plate and the reservoir lid forms an intimate fit on top of the well plate;
• wherein the chamber insert comprises a plurality of well inserts, wherein each well insert has a media supply compartment and a media collection compartment in fluid communication with one another and wherein a bottom of the well insert is open;
• wherein the reservoir lid comprises a plurality of media supply wells and a plurality of media collection ports;
• and wherein when the perfusion system is assembled, each of the media supply wells is in fluid communication with a corresponding one of the plurality of media supply compartments and each of the media collection ports is in fluid communication with a corresponding media collection compartment.