US 12,729,357 B2 · Microscale bioreactor system for and method of providing cell culture growth conditions in a small-volume vessel

Abstract

A microscale bioreactor system for and method is disclosed for providing improved cell culture growth conditions in a small-volume vessel. For example, a microbioreactor system is provided that may include a small-volume vessel and wherein the small-volume vessel may include a field of actuatable surface-attached microposts. Further, the microbioreactor system may include an actuation mechanism for actuating the surface-attached microposts into movement. In some embodiments, the surface-attached microposts may be functionalized with, for example, activation signals for converting standard T-cells in a growth media to activated T-cells. Further, a method of using the microbioreactor system for providing cell culture growth conditions including enhanced oxygenation and nutrients distribution in a small-volume vessel is provided.

Claims14

Dependent claims

A method of maintaining cell culture growth conditions within a small-volume bioreactor, the method comprising:

(a) placing cell culture media in wells of a small-volume well layer of the small-volume bioreactor comprising an array of wells, each well having a top opening and a bottom opening;
(b) placing the cell culture media in contact with an active surface layer of the small-volume bioreactor having an array of actuable surface-attached microposts, wherein the microposts extend into the bottom openings of the array of wells of the well layer;
(c) actuating movement of the surface-attached microposts to create a mixing action whereby the mixing action maintains cell culture growth conditions within the small-volume bioreactor;
and
(d) measuring an oxygen transfer coefficient (kLa) of the cell culture media to quantify a mixing efficiency of the surface-attached microposts.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

18/014,967Filed 7 Jul 2021 · us provisional application

Priority

7 Jul 2020

Published

US 2023/0257689 A117 Aug 2023

Granted

8 Sep 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

C12M 23/12
Form or structure of the vessel: well or multiwell plates
B01F 33/3038
Micromixers: using ciliary stirrers to move or stir the fluids
C12M 27/02
Means for mixing, agitating or circulating fluids in the vessel: stirrer or mobile mixing elements
B01F 2101/44
Mixing characterised by the nature of the mixed materials or by the application field: mixing of ingredients for microbiology, enzymology, in…

Microscale bioreactor system for and method of providing cell culture growth conditions in a small-volume vessel

Life of this patent

Microscale bioreactor system for and method of providing cell culture growth conditions in a small-volume vessel

Life of this patent

Abstract

Claims14