US 12,729,238 B2 · Nanodisc-specific antigen-binding chimeric proteins

Abstract

The present invention relates to the field of structural biology. More specifically, the present invention relates to an antigen-binding chimeric protein, called a MegaBody™, specifically binding a nanodisc, more specifically a membrane-scaffold protein (MSP)n which may be part of the nanodisc. The invention further provides for methods and uses of said nanodisc-specific antigen-binding chimeric proteins in three-dimensional high-resolution structural analysis of membrane proteins assembled within nanodiscs. The MSP-binding MegaBodies of the invention provide for a generic tool in membrane protein structural biology, more particular in Cryo-EM, by reducing preferred particle orientation of nanodiscs and of the entrapped target membrane proteins.

Claims11

Dependent claims

An antigen-binding domain that specifically binds a membrane-scaffold protein (MSP) or an MSP variant, wherein the antigen-binding domain comprises an immunoglobulin single variable domain (ISVD), and the ISVD comprises an amino acid sequence selected from:

(i) any one of the amino acid sequences of SEQ ID NOs:
37-50;
or
(ii) a homologue thereof that is at least 95% identical to any one of the amino acid sequences of SEQ ID NOs:
37-50, provided the homologue's CDRs (Complementarity Determining Regions) are identical to the CDRs present in that same one of the amino acid sequences of SEQ ID NOs:
37-50.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

17/770,776Filed 21 Oct 2020

Priority

EP 1920441221 Oct 2019

Published

US 2022/0380456 A11 Dec 2022

Granted

8 Sep 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification6 codes

C07K 16/28
Against material from animals or humans: against receptors, cell surface antigens or cell surface determinants
C07K 14/775
From animals; from humans: apolipopeptides
C12N 15/63
Recombinant DNA-technology: introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C07K 2317/31
Characterized by aspects of specificity or valency: multispecific

Nanodisc-specific antigen-binding chimeric proteins

Life of this patent

Nanodisc-specific antigen-binding chimeric proteins

Life of this patent

Abstract

Claims11