US 12,729,222 B2 · Enhanced peptide constructs for albumin binding

Abstract

A synthetic peptide construct comprises an amino acid sequence-RLIEDICLPRWGCLWEDD (SEQ ID NO: 2) (also known as “GLYCLOK”), a flexible, hydrophilic glycine-serine (Gly-Ser) linker, and a polypeptide chain between 25 to 70 amino acids in length, which incorporates an albumin-binding moiety with a dissociation constant (Kd) to albumin of less than 20 nanomolar (nM). This construct may include the sequence RLIEDICLPRWGCLWEDD (SEQ. ID NO: 2) within its terminal 25 amino acids. The albumin-binding moiety may be cyclic, with a disulfide bond formed by at least one cysteine residue. The constructs is used as an agonist to modulate receptor interactions in vivo, enhancing therapeutic efficacy and extending circulating half-life in mammals, including humans. The peptides exhibit significant binding to albumin, enhancing bioavailability and therapeutic duration.

Claims16

Dependent claims

A synthetic peptide construct comprising:

a polypeptide chain between 5 to 200 amino acids in length, inclusive, said polypeptide chain having the internal amino acid or branched sequence or terminal sequence including DICLPRWGCLW (SEQ ID NO:
1) as part of
a novel albumin binding moiety covalently bound to said polypeptide chain, said albumin binding moiety comprising a flexible and hydrophilic glycine-serine (Gly-Ser) linker of SEQ ID NO:
5,
having a binding dissociation constant (Kd) to human-albumin of less than 30 nanomolar (nM).

2 more elements

Dependencies are inferred from the claim text.

Enhanced peptide constructs for albumin binding

Life of this patent

Enhanced peptide constructs for albumin binding

Life of this patent

Abstract

Claims16