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US 12,729,217 B2Granted
Method of synthesizing single-stranded nucleotide sequence, blocked nucleoside triphosphates and related methods
AssigneeAgency for Science, Technology and Research
InventorsJean-Alexandre Richard, Xi Lin
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Abstract
There is provided a method of synthesizing a single-stranded nucleotide sequence, the method comprising adding a blocked nucleoside triphosphate to an initiator nucleotide sequence to incorporate a corresponding blocked nucleotide thereto in the presence of a polymerase, wherein the blocked nucleoside triphosphate has one of the general formulae (I), (II), (III), (IV), (V) and (VI).
Claims18
A method of synthesizing a single-stranded nucleotide sequence, the method comprising:
- (i) adding a blocked nucleoside triphosphate to an initiator nucleotide sequence and incorporating a corresponding blocked nucleotide thereto in the presence of a polymerase,
- wherein the blocked nucleoside triphosphate has one of the general formulae (III) and (VI):
- wherein
- m=0 to 20;
- Rz is H or OH;
- R8, R16, R17 and R18 are independently selected from hydrogen, alkyl, alkenyl, aryl or heteroaryl;
- X is a heteroatom selected from O, S or NH;
- and
- Y1 and Y2 are independently selected from S or Se.
6 more elements
Dependencies are inferred from the claim text.