Gravity flow micro-physiological article and determining a physiological response to a drug

A gravity flow micro-physiological article for determining a physiological response to a drug, the gravity flow micro-physiological article comprising:

• a substrate;
• a first supply chamber disposed on the substrate configured to contain a first fluid volume and to direct a first supply fluid flow;
• a second supply chamber disposed on the substrate configured to contain a second fluid volume and to direct a second supply fluid flow, such that the first supply fluid flow from the first supply chamber is parallel to the second supply fluid flow from the second supply chamber;
• a mixing chamber in fluid communication with the first supply chamber and the second supply chamber the mixing chamber defining a unitary internal volume structurally arranged to:
• receive the first supply fluid flow from the first supply chamber;
• receive the second supply fluid flow from the second supply chamber;
• and combine the first supply fluid flow and the second supply fluid flow to form a combined fluid flow within the unitary internal volume;
• a liquid divider in fluid communication with the mixing chamber, wherein the liquid divider comprises a physical divider wall disposed therein that structurally partitions the liquid divider into a first return path and a second return path:
• wherein the liquid divider:
• receives the combined fluid flow from the mixing chamber;
• and divides the combined fluid flow via the physical divider wall into a first divided fluid flow and a second divided fluid flow, wherein the liquid divider is in fluid communication with the first supply chamber and the second supply chamber such that:
• the first supply chamber receives the first divided fluid flow from the liquid divider;
• and the second supply chamber receives the second divided fluid flow from the liquid divider;
• and a plurality of flow channels fluidically interconnecting the supply chambers, the mixing chamber, and the liquid divider, and providing fluid flow therebetween, such that the combined fluid flow is recirculated through the flow channels and returned to at least one of the supply chambers in an absence of a fluidic actuator or pump, wherein the first supply chamber, the second supply chamber, the mixing chamber, and the flow channels are open and directly exposed to the atmosphere;
• and the gravity flow micro-physiological article communicates fluid among the first supply chamber, the second supply chamber, the mixing chamber, and the flow channels due to gravity as the gravity flow micro-physiological article does not comprise a fluidic actuator or a pump.