Microfluidic chip and its production

A microfluidic chip production process characterized by comprising;

• A. Production of a 3 dimensionally designed bottom chip (A3) and an upper lid (A4) chip components, with injection moulding (A2) technique
• B. Cleaning of the bottom chip (A3) surface with an isopropyl alcohol solution (B4) in a primary immersion tank (B3) consisting of at least one basket (B2) in which at least one chip cartridge system (B1) is located and drying them with a ventilation drying system (D1);
• C. Activation of the bottom chip (A3) surface with sodium hydroxide solution (E2) by immersing them into a secondary immersion tank (E1) consisting of at least one basket (B2) in which at least one chip cartridge system (B1) is located and drying them with a ventilation drying system (D1);
• D. The first modification of the bottom chip (A3) surface with Polyethyleneimine solution (H2) by immersing them into a third immersion tank (H1) which consists of at least one basket (B2) in which at least one chip cartridge system (B1) is located and drying them with a ventilation drying system (D1);
• E. The second modification of the bottom chip (A3) surface with glutaraldehyde solution (K2) by immersing them into a fourth immersion tank (K1) which consists of at least one basket (B2) in which at least one chip cartridge system (B1) is located and drying them with a ventilation drying system (D1);
• F. With a microdispensary (N1), dripping the protein solution (N2) on chips which were twice modified, washed and dried;
• G. Incubating the bottom chips (A3) on which the protein solution is dripped, within an incubator system with diffuser humidification (O1) which provides humidity;
• H. Washing the bottom chip (A3) surface with a PBST solution (P2) by immersing them into a fifth immersion tank (P1) which consists of at least one basket (B2) in which at least one chip cartridge system (B1) is located and drying them with a ventilation drying system (D1);
• I. Blocking the bottom chip (A3) with bovine serum albumin (BSA) solution (T2) by immersing them into a sixth immersing tank (T1) which consists of at least one basket (B2) in which at least one chip cartridge system (B1) is located and drying them with a ventilation drying system (D1), resulting in protein-coated bottom chips (V2);
• J. With a vacuum plate system (Y1) connected to a vacuum pump and line (Y2), through a laser cutting device with a vacuum (Y4), following the double-sided tape (Y3) cutting, the doublesided cut tape (Z1) is produced;
• K. By attaching the upper lid (A4) to one of the sides of the double-sided cut tape (Z1), making the double-sided tape lid (AB1);
• L. By attaching the double-sided tape lid (AB1) with the installation mechanism (AB2) and placing the protein-coated bottom chip (V2), making the microfluidic chip (AB3).