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US 12,728,172 B2Granted
Sperm nuclei and methods of their manufacture and use
AssigneeInguran, LLC
InventorsKenneth Michael Evans, Thomas B. Gilligan
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Abstract
The invention encompasses a rapid and safe preparation method of sperm nuclei, improved sperm nuclei and a method of using the improved sperm nuclei to calibrate a flow cytometer with higher accuracy.
Claims5
A method of processing sperm cells comprising
- providing an unsorted sperm cell sample;
- combining a DNA selective dye and an aggregation-reducing compound with the unsorted sperm cell sample to create a sperm cell mixture;
- and
- sonicating the sperm cell mixture to create stained sperm nuclei, wherein the sperm cell mixture has a temperature of 45° C. or greater during the step of sonication.
1 more element
Dependencies are inferred from the claim text.