Mangosteen pericarp extractand process for its preparation thereof

A method for inhibiting weight gain, insulin resistance, and fat accumulation in adipose and muscle tissue induced by a high fat diet in a human in need thereof consisting essentially of administering to the human in need thereof a therapeutically effective amount of an extract of mangosteen pericarp, wherein the extract of the mangosteen pericarp extract is made by a process consisting essentially of:

• a) drying and fragmenting a mangosteen pericarp to obtain a fragment of the mangosteen pericarp;
• b) soaking the fragment of the mangosteen pericarp in at least 70% ethanol to yield a mangosteen pericarp extract and holding it at room temperature;
• c) filtering the mangosteen pericarp extract to obtain a mangosteen pericarp organic solvent extract, wherein the ratio of the weight to volume of the fragment of mangosteen pericarp and the organic solvent is 1:2 to 1:20;
• d) soaking and heating the fragment of mangosteen pericarp organic solvent extract of step c) in an aqueous solution and holding it at room temperature;
• e) filtering it to obtain a mangosteen pericarp aqueous extract and a mangosteen pericarp residue, wherein the ratio of the weight to volume of the fragment of mangosteen pericarp and the aqueous solution is 1:10 to 1:20;
• f) concentrating the mangosteen pericarp organic solvent extract and the mangosteen pericarp aqueous extract to obtain a mangosteen pericarp organic solvent concentrate and a mangosteen pericarp aqueous concentrate;
• g) adding the mangosteen pericarp organic solvent concentrate to an aqueous solution;
• h) homogenizing it, holding it at room temperature;
• i) separating it into a first upper layer aqueous solution and a first lower layer precipitate;
• j) isolating the first upper layer aqueous solution and adding ethanol to dissolve the first lower layer precipitate;
• k) obtaining a mangosteen pericarp ethanol extract by filtration;
• l) adding alkane solvent with a volume ratio of 1:1 to 1:10 into the mangosteen pericarp ethanol extract and mixing it with a vortex mixer, holding it at room temperature;
• m) separating it into an upper layer alkane solvent and a lower layer ethanol filtrate;
• n) heating and concentrating the lower layer ethanol filtrate in water bath to obtain a lower layer ethanol concentrated filtrate;
• o) adding and mixing aqueous solution with a volume ratio of 1:1 to 3:1 into the lower layer ethanol concentrated filtrate, holding it at low temperature;
• p) separating it into a second upper layer aqueous solution and a second lower layer precipitate;
• and q) fragmenting, air-lay drying and grinding the second lower layer precipitate to obtain the mangosteen pericarp ethanol extract.