Trojan circulating tumor cells
AssigneeAstrin Biosciences, Inc.
InventorJayant Parthasarathy
Life of this patent
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Abstract
Provided herein are compositions and methods for engineering treatment resistant circulating tumor cells to generate immune stimulating or anti-cancer exosomes, which can be infused into a patient to self-home to the primary tumor and metastatic lesions. Also provided are compositions and methods for forming a population of engineered exosome-loaded dendritic cells, which can be infused into the patient to activate a systemic immune response. The compositions and methods allow for infiltration into solid tumors using “trojan” cells that are naturally adapted and mechanistically engineered to evade patients' defenses.
Claims20
A method of treating a cancer patient comprising:
- (a) extracting circulating tumor cells from the cancer patient to produce a population of isolated circulating tumor cells;
- (b) isolating a population of treatment resistant circulating tumor cells from the population of isolated circulating tumor cells;
- (c) genetically engineering the treatment resistant circulating tumor cells to generate immune stimulating or anti-cancer exosomes to produce a population of genetically engineered treatment resistant circulating tumor cells;
- (d) administering the genetically engineered treatment resistant circulating tumor cells to the cancer patient.
A method of treating a cancer patient comprising:
- (a) extracting circulating tumor cells from a cancer patient to produce a population of isolated circulating tumor cells;
- (b) isolating a population of treatment resistant circulating tumor cells from the population of isolated circulating tumor cells;
- (c) harvesting exosomes from the population of isolated treatment resistant circulating tumor cells to produce a population of isolated treatment resistant circulating tumor cell exosomes;
- (d) incubating the population of isolated treatment resistant circulating tumor cell exosomes with dendritic cells to form a population of exosome-loaded dendritic cells;
- and
- (e) infusing the population of exosome-loaded dendritic cells into a cancer patient in need thereof.
Dependencies are inferred from the claim text.